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Multimeric oncolytic adenoviruses, which contain more than one wild type adenovirus to improve therapeutic efficacy, are being developed as new anticancer treatments. Phosphorylation of the serine/threonine protein kinase Akt is strongly regulated by growth factors, and Akt activation is closely linked to cellular transformation, tumour formation, and tumour progression. In the present study, we have examined the effect of the multimeric oncolytic adenovirus Delta-24-RGD expressing the mutant form of E1A, which is defective in interaction with the retinoblastoma protein, on Akt phosphorylation and cellular proliferation in a colon carcinoma cell line, designated CACO-2. An in vitro high-cell-density colony formation assay in soft agar was also performed to investigate the effect of Delta-24-RGD on tumour cell transformation. Delta-24-RGD caused a significant decrease in the phosphorylation of Akt both in CACO-2 cells infected at high cell density (approximately 10(6) cells/T-flask) and at low cell density (approximately 10(4) cells/T-flask). The adenovirus also reduced the cellular proliferation of the CACO-2 cells, especially after the cells had been incubated at a high cell density, and markedly inhibited the formation of high-cell-density colonies of the CACO-2 cells in soft agar. These findings indicate that Delta-24-RGD is effective against colon cancer both in vitro and in vivo and might have the potential to become a potent therapeutic tool for human colon cancer.Q:

Loading images from a specific folder in python

I want to load images from a folder into the img variable. For example I have 1000 images in a folder. I want to load from 1001 to 2000 to img.

A:

If you know what directories you have, then use os.

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